| Population name | Wulandari |
| Genome | not specified |
| Consortium | Universities based in Indonesia and the UK |
| Super population | EAS |
| Population description | Patients with moderate and severe COVID-19 (n = 62, 65.3%) were hospitalised in Dr Soetomo General Academic Hospital, Surabaya, Indonesia, whilst 33 patients (34.7%) with asymptomatic or mild symptoms were treated in Indrapura KOGABWILHAN II Hospital, Surabaya, Indonesia. |
| Population origin | Indonesia |
| Case population size | 95 |
| Control population size | none |
| Comorbidities | Diabetes(n=21), CVD (25), Liver disease (13), Kidney disease (5), Lung disease (3) |
| Mean / median age | 44.7 +/- 1.3 |
| Sex | 60 male 35 female |
| Severity | Susceptibility |
| Sample source | Nasopharyngeal swab |
| Method | DNA extraction was performed using the QIAamp® Blood DNA Midi kit and DNA concentrations determined using a microvolume spectrophotometer. The TMPRSS2 polymorphism was detected using a TaqMan SNP genotyping assay. Genotyping was performed RT-PCR with VIC and FAM fluorescent reporters to indicate allelic discrimination. |
| Bioinformatics | Statistical analyses were performed using the IBM SPSS Statistics Software ver. 23 (IBM Corp.) or GraphPad Prism ver. 8 (GraphPad Software, LLC). A chi-squared test was used to examine the Hardy–Weinberg equilibriums and to determine the association between categorical variables in the cross-tabulation data. ANOVA with post hoc multiple comparisons was used to analyse numerical data. A P value less than 0.05 was considered to be statistically significant. |
| Imputation details | NA |
| Limitations | a. Small sample size with larger studies required for validation, b. A Ct value was used to determine viral load which can only provide an estimate, and c. The effect of the variant on protein function needs to be performed. |