| Population name | Gomez |
| Genome | not specified |
| Consortium | Hospitals, Universities and Institutes in Spain |
| Super population | EUR |
| Population description | Cohort requiring hospitalisation with 67 patients in need of critical care support, including high-flow oxygen, positive-pressure ventilation (either invasive or non-invasive) or vasoactive drugs. |
| Population origin | Asturias, Northern Spain |
| Case population size | 204 |
| Control population size | 536 |
| Comorbidities | Hypertension (48%), diabetes (18%), hypercholesterolaemia (34%) |
| Mean / median age | 64.77 |
| Sex | 61% male |
| Severity | Severe |
| Sample source | Nasopharyngeal swab |
| Method | The I/D polymorphism (rs4646994) in intron 16 of the ACE gene was genotyped by PCR followed by agarose gel electrophoresis to visualise the two alleles. For the ACE2 rs2285666 A/G SNP the PCR fragments were digested with the restriction enzyme AluI and electrophoresis on agarose gels. The ACE2 coding exons of 60 male patients (30 severe and 30 nonsevere) were amplified with primers designated from exon flanking introns. These fragments were sequenced with Sanger BigDye chemistry in a capillary A |
| Bioinformatics | The statistical analysis was performed with the R-project free software (www.rproject.org). The logistic regression (linear generalized model, LGM) was used to compare mean values and frequencies between the groups. |
| Imputation details | NA |
| Limitations | a. Small sample size and low number of severe female cases limited the statistical interpretation of the significant and non-significant associations, b. Individuals exposed but asymptomatic were not studied |